Measurement
Part:BBa_K299023
Designed by: Anna Olchowik Group: iGEM10_Warsaw (2010-10-16)
RBS measurement device pT7 B0033 GFP
This part is licensed under
Creative BioCommons
We planned to use this device to determine BBa_B0033 strength relative to BBa_B0034 using cell-free system.
Authors
Clonning:
- Ania Puławska
- Milena Bażlekowa
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Construct design
The device consists of BBa_I719005 T7 promoter followed by BBa_B0033 community RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein. Device was created by cloning BBa_I13401 behind ExpressIt! vector containing appropriate RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 692
[edit]
Categories
Parameters
n/a | RBS measurement device pT7 B0033 GFP |